Mehran Salehpour

Mehran Salehpour
Associate Professor
Department of Engineering Sciences
Uppsala University
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Biomedical applications of Accelerator Mass Spectrometry

Accelerator Mass Spectrometry (AMS) is an ultra-sensitive method where isotopic ratio measurements provide fundamental data in a variety of disciplines, spanning from archaeology, geology and environmental sciences to dating of cosmogenic objects and solar activity chronology. We are applying AMS to the biomedical sciences where the most prevalent isotope, 14C is used to reveal some underlying processes taking place in the human body.

In the Human Regenerative Map project, we apply AMS to the analysis of human DNA from miscellaneous cell nuclei, revealing the age of formation and therefore level of regeneration for different cell types. Any information on the regenerative properties of the cells is pertinent to the choice of therapeutic and diagnostic approach for future research. We are developing measurement methods to address the requirements set forth, by among others, small sample amounts available after purification steps.

Other biomedical applications of interest include:

Obstructive tissue dating: Determination of build-up time of obstructive tissues such as senile and carotid plaques leading to pathological condition.

Microdosing: Also referred to as the Human Phase-0 Clinical Trails: Pharmaceutical drugs are applied directly to humans at a very low dose (µg) and at an early stage to obtain pharmacokinetic data (Absorption, Distribution, Metabolism, Excretion) to shorten the drug development time. The method is also applied to proteins and polypeptides as drug candidates.

 

Selected publications:

Salehpour M,Possnert G, Bryhni H. Subattomolar sensitivity in biological Accelerator mass Spectrometry, Anal Chem. 2008; 80: 3515.

Accelerator Mass Spectrometry of small biological samples Salehpour M, Forsgard N and Possnert G. Rapid Communications in Mass Spectrometry. 2008; 22: 3928.

FemtoMolar Measurements using Accelerator Mass Spectrometry Salehpour M, Forsgard N and Possnert G. Rapid Communications in Mass Spectrometry. 2009; 23: 557.

Biological Accelerator Mass Spectrometry at Uppsala University Salehpour M, Possnert G, Bryhni H, Palminger-Hallén I, Ståhle L. Applied Radiation and Isotopes 2008; 67: 495.

Biological AMS at Uppsala University Salehpour M, Forsgard N and Possnert G, Accepted for publication in Nuclear Instruments and Methods B 2009. DOI: 10.1016/j.nimb.2009.10.055

Accelerator Mass Spectrometry in the Attomolar Concentration Range for 14C-labelled Biologically Active Compounds in Complex Matrixes. Forsgard N, Salehpour M and Possnert G. Accepted for publication in J. Anal. At. Spectrom. 2009. DOI:10.1039/B906433H